Open AccessCrystallization and preliminary crystallographic studies of the HA3 subcomponent of the type B botulinum neurotoxin complex
Author(s) -
Nishimura Kohsuke,
Kitadokoro Kengo,
Takegahara Yuki,
Sugawara Yo,
Matsumura Takuhiro,
Karatani Hajime,
Fujinaga Yukako
Publication year - 2011
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309111027412
Subject(s) - crystallization , crystallography , toxin , chemistry , botulinum neurotoxin , neurotoxin , stereochemistry , biochemistry , organic chemistry
The haemagglutinin subcomponent HA3 of the type B botulinum neurotoxin complex, which is important in toxin absorption from the gastrointestinal tract, has been expressed, purified and subsequently crystallized in two crystal forms at different pH values. Form I belonged to space group R 32, with unit‐cell parameters a = b = 357.4, c = 249.5 Å, α = β = 90, γ = 120°. Form II belonged to space group I 4 1 32, with unit‐cell parameters a = b = c = 259.0 Å, α = β = γ = 90°. Diffraction data were collected from these crystals to a resolution of 3.0 Å for both form I and form II.