Open AccessCrystallization and preliminary X‐ray analysis of a glucansucrase from the dental caries pathogen Streptococcus mutans
Author(s) -
Ito Keisuke,
Ito Sohei,
Shimamura Tatsuro,
Kawarasaki Yasuaki,
Abe Keiko,
Misaka Takumi,
Kobayashi Takuya,
Iwata So
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110029714
Subject(s) - streptococcus mutans , microbiology and biotechnology , pathogen , crystallization , dentistry , medicine , chemistry , biology , bacteria , genetics , organic chemistry
Glucansucrases encoded by Streptococcus mutans play essential roles in the synthesis of sticky dental plaques. Based on amino‐acid sequence similarity, glucansucrases are classified as members of glycoside hydrolase family 70 (GH 70). Data on the crystal structure of GH 70 glucansucrases have yet to be reported. Here, the GH 70 glucansucrase GTF‐SI from S. mutans was overexpressed in Escherichia coli strain BL21 (DE3), purified to homogeneity and crystallized using the hanging‐drop vapour‐diffusion method. Orthorhombic GTF‐SI crystals belonging to space group P 2 1 2 1 2 were obtained. A diffraction data set was collected to 2.1 Å resolution.