Open AccessThe Corynebacterium glutamicum aconitase repressor: scratching around for crystals
Author(s) -
GarcíaNafría Javier,
Baumgart Meike,
Bott Michael,
Wilkinson Anthony J.,
Wilson Keith S.
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110029015
Subject(s) - corynebacterium glutamicum , repressor , aconitase , scratching , chemistry , microbiology and biotechnology , crystallography , biochemistry , materials science , biology , gene , enzyme , composite material , gene expression
Imperfections on the surfaces of crystallization containers are known to influence crystal formation and are thought to do so by helping to overcome the nucleation barrier. The intentional creation of imperfections has been widely applied to induce crystallization of small molecules, but has not been reported for protein crystallization. Here, the crystallization and preliminary X‐ray analysis of the TetR‐type aconitase repressor are reported. This regulator was the first transcription factor to be identified in the regulation of the tricarboxylic acid cycle in Corynebacterium glutamicum , an organism that is of special industrial interest and is an emerging model organism for Corynebacterineae. Successful crystallization involved introducing manual scratches on the surface of standard commercial plates, which led to a substantial improvement in crystal nucleation and quality.