Open AccessCrystallization and preliminary X‐ray study of the d ‐altritol oligonucleotide GTGTACAC
Author(s) -
Ovaere Margriet,
Van Aerschot Arthur,
Abramov Mikhail,
Herdewijn Piet,
Van Meervelt Luc
Publication year - 2010
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309110007050
Subject(s) - crystallization , oligonucleotide , materials science , x ray , crystallography , chemistry , physics , optics , dna , biochemistry , organic chemistry
In altritol nucleic acids (ANAs), the natural five‐membered ribose ring of RNA is replaced by the six‐membered d ‐altritol ring. ANAs are good candidates to act as siRNAs in the RNA‐interference pathway. Crystals of the fully modified altritol self‐complementary octamer GTGTACAC were grown by the hanging‐drop vapour‐diffusion technique at 289 K. Diffraction data were recorded on SLS beamline X06DA and processed to 3.0 Å resolution. The crystals belonged to the hexagonal space group P 6 1 22 or P 6 5 22, with unit‐cell parameters a = 25.05, c = 117.58 Å.