Open AccessCrystallization and preliminary X‐ray diffraction analysis of a putative two‐domain‐type laccase from a metagenome
Author(s) -
Komori Hirofumi,
Miyazaki Kentaro,
Higuchi Yoshiki
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309109002462
Subject(s) - crystallization , crystallography , trimer , synchrotron radiation , materials science , x ray , diffraction , resolution (logic) , analytical chemistry (journal) , chemistry , physics , dimer , optics , chromatography , organic chemistry , artificial intelligence , computer science
A putative two‐domain‐type laccase retrieved from a metagenome was successfully crystallized using the sitting‐drop vapour‐diffusion method. Data were collected to a resolution of 1.7 Å at 100 K using synchrotron radiation. The crystal belonged to space group P 2 1 2 1 2 1 , with unit‐cell parameters a = 74.67, b = 100.95, c = 124.11 Å. The self‐rotation function showed the presence of a noncrystallographic threefold axis in the structure. The presence of one trimer in the asymmetric unit yielded a Matthews coefficient ( V M ) of 2.05 Å 3 Da −1 and a solvent content of 40%.