Open AccessCrystallization and diffraction of an Lhx4–Isl2 complex
Author(s) -
Gadd Morgan S.,
Langley David B.,
Guss J. Mitchell,
Matthews Jacqueline M.
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108043431
Subject(s) - crystallization , crystallography , materials science , diffraction , chemistry , physics , optics , organic chemistry
A stable intramolecular complex comprising the LIM domains of the LIM‐homeodomain protein Lhx4 tethered to a peptide region of Isl2 has been engineered, purified and crystallized. The monoclinic crystals belonged to space group P 2 1 , with unit‐cell parameters a = 46.8, b = 88.7, c = 49.9 Å, β = 111.9°, and diffracted to 2.16 Å resolution.