Open AccessExpression, purification, crystallization and preliminary X‐ray diffraction analysis of the soluble domain of PPA0092, a putative nitrite reductase from Propionibacterium acnes
Author(s) -
Nojiri Masaki,
Shirota Felicia,
Hira Daisuke,
Suzuki Shinnichiro
Publication year - 2009
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108040207
Subject(s) - nitrite reductase , crystallization , nitrite , propionibacterium acnes , chemistry , reductase , domain (mathematical analysis) , biochemistry , biology , nitrate reductase , enzyme , organic chemistry , bacteria , genetics , nitrate , mathematics , mathematical analysis
The soluble domain (residues 483–913) of PPA0092, a putative copper‐containing nitrite reductase from Propionibacterium acnes KPA171202, has been overexpressed in Escherichia coli . The purified recombinant protein was crystallized using the hanging‐drop vapour‐diffusion method. X‐ray diffraction data were collected and processed to a maximum resolution of 2.4 Å. The crystal belonged to space group P 2 1 3, with unit‐cell parameters a = b = c = 108.63 Å. Preliminary diffraction data show that one molecule is present in the asymmetric unit; this corresponds to a V M of 2.1 Å 3 Da −1 .