Open AccessEffect of leucine‐to‐methionine substitutions on the diffraction quality of histone chaperone SET/TAF‐Iβ/INHAT crystals
Author(s) -
Senda Miki,
Muto Shinsuke,
Horikoshi Masami,
Senda Toshiya
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108028704
Subject(s) - methionine , leucine , histone , crystallography , chemistry , chaperone (clinical) , biochemistry , amino acid , dna , medicine , pathology
One of the most frequent problems in crystallization is poor quality of the crystals. In order to overcome this obstacle several methods have been utilized, including amino‐acid substitutions of the target protein. Here, an example is presented of crystal‐quality improvement by leucine‐to‐methionine substitutions. A variant protein with three amino‐acid substitutions enabled improvement of the crystal quality of the histone chaperone SET/TAF‐Iβ/INHAT when combined with optimization of the cryoconditions. This procedure improved the resolution of the SET/TAF‐Iβ/INHAT crystals from around 5.5 to 2.3 Å without changing the crystallization conditions.