Open AccessPurification, crystallization and preliminary crystallographic analysis of a 6‐pyruvoyltetrahydropterin synthase homologue from Esherichia coli
Author(s) -
Seo Kyung Hye,
Kim Hye Lim,
Park Young Shik,
Jeon Che Ok,
Lee Kon Ho
Publication year - 2008
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309108000626
Subject(s) - crystallography , crystallization , hexagonal crystal system , resolution (logic) , crystal (programming language) , molecule , diffraction , molecular replacement , group (periodic table) , materials science , chemistry , crystal structure , physics , optics , organic chemistry , artificial intelligence , computer science , programming language
6‐Pyruvoyltetrahydropterin synthase from E. coli (ePTPS) has been crystallized using the hanging‐drop vapour‐diffusion method. Hexagonal‐ and rectangular‐shaped crystals were obtained. Diffraction data were collected from the hexagonal and rectangular crystals to 3.0 and 2.3 Å resolution, respectively. The hexagonal plate‐shaped crystals belonged to space group P 321, with unit‐cell parameters a = b = 112.59, c = 68.82 Å, and contained two molecules in the asymmetric unit. The rectangular crystals belonged to space group I 222, with unit‐cell parameters a = 112.76, b = 117.66, c = 153.57 Å, and contained six molecules in the asymmetric unit. The structure of ePTPS in both crystal forms has been determined by molecular replacement.