Open AccessCrystallization and preliminary X‐ray diffraction analysis of the full‐length Mg 2+ transporter MgtE
Author(s) -
Hattori Motoyuki,
Tanaka Yoshiki,
Fukai Shuya,
Ishitani Ryuichiro,
Nureki Osamu
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107032332
Subject(s) - thermus thermophilus , crystallization , crystallography , synchrotron radiation , transporter , diffraction , chemistry , escherichia coli , gene , biochemistry , physics , optics , organic chemistry
The MgtE family of Mg 2+ transporters are ubiquitously conserved in all three domains. The genes encoding full‐length MgtE from seven different species were cloned. Three of the seven MgtE transporters were overexpressed and purified for use in crystallization trials. Only Thermus thermophilus MgtE was successfully crystallized using the sitting‐drop vapour‐diffusion method. Selenomethionine‐substituted (SeMet) crystals were obtained by cross‐microseeding using the native microcrystals. The SeMet crystals diffracted X‐rays to 3.5 Å resolution using synchrotron radiation and belong to space group C 222 1 , with unit‐cell parameters a = 118.3, b = 134.9, c = 366.2 Å. Structure determination is in progress.