Open AccessA high‐resolution structure of ligand‐free human glutamate carboxypeptidase II
Author(s) -
Barinka Cyril,
Starkova Jana,
Konvalinka Jan,
Lubkowski Jacek
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910700379x
Subject(s) - glutamate receptor , resolution (logic) , carboxypeptidase a , ligand (biochemistry) , carboxypeptidase , chemistry , materials science , crystallography , biochemistry , computer science , artificial intelligence , receptor , enzyme
Human glutamate carboxypeptidase II (GCPII; EC 3.4.17.21) is an established marker for prostate‐cancer diagnosis as well as a candidate therapeutic target for the treatment of diverse pathologies that involve glutamatergic transmission. Structural data on GCPII are thus valuable for the design and optimization of GCPII‐specific inhibitors and diagnostic probes. The currently available structure of ligand‐free GCPII was refined to a resolution of 3.5 Å. This work reports the structure of the protein refined to 1.65 Å resolution, with crystallographic values of R = 0.207 and R free = 0.228. The new structure extends the resolution appreciably and the new model based on this data shows significant differences when compared with the previously published model.