Open AccessCrystallization and preliminary X‐ray diffraction study of glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis
Author(s) -
Katsumi Ryota,
Koga Yuichi,
You DongJu,
Matsumura Hiroyoshi,
Takano Kazufumi,
Kanaya Shigenori
Publication year - 2007
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s1744309107001388
Subject(s) - thermococcus , glycerol , archaea , crystallization , crystallography , chemistry , materials science , biochemistry , organic chemistry , gene
Glycerol kinase from the hyperthermophilic archaeon Thermococcus kodakaraensis was crystallized and preliminary crystallographic studies of the crystals were performed. Crystals were grown at 293 K by the sitting‐drop vapour‐diffusion method. Native X‐ray diffraction data were collected to 2.4 Å resolution using synchrotron radiation at station BL44XU of SPring‐8. The crystal belongs to the rhombohedral space group R 3, with unit‐cell parameters a = b = 217.48, c = 66.48 Å. Assuming the presence of two molecules in the asymmetric unit, the V M value was 2.7 Å 3 Da −1 and the solvent content was 54.1%. The protein was also cocrystallized with substrates and diffraction data were collected to 2.7 Å resolution.