Open AccessPurification, partial characterization and preliminary X‐ray diffraction analysis of a mannose‐specific lectin from Cymbosema roseum seeds
Author(s) -
Cavada Benildo S.,
Marinho Emmanuel S.,
Souza Emmanuel P.,
Benevides Raquel G.,
Delatorre Plínio,
Souza Luis A. G.,
Nascimento Kyria S.,
Sampaio Alexandre H.,
Moreno Frederico B. M. B.,
Rustiguel Joane K. R.,
Canduri Fernanda,
De Azevedo Walter F.,
Debray Henri
Publication year - 2006
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910600371x
Subject(s) - lectin , characterization (materials science) , mannose , mannan binding lectin , chemistry , biochemistry , materials science , nanotechnology
A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour‐diffusion method using a precipitant solution consisting of 0.1 M Tris–HCl pH 7.8, 8%( w / v ) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 Å resolution at a synchrotron‐radiation source. CRL crystals are orthorhombic, belonging to space group P 2 1 2 1 2 1 . Crystallographic refinement and full amino‐acid sequence determination are in progress.