
Crystallization and preliminary X‐ray analysis of the Man(α1‐2)Man‐specific lectin from Bowringia mildbraedii in complex with its carbohydrate ligand
Author(s) -
GarciaPino Abel,
Loris Remy,
Wyns Lode,
Buts Lieven
Publication year - 2005
Publication title -
acta crystallographica section f
Language(s) - English
Resource type - Journals
ISSN - 1744-3091
DOI - 10.1107/s174430910502854x
Subject(s) - lectin , crystallization , crystallography , nucleation , monomer , chemistry , resolution (logic) , synchrotron radiation , concanavalin a , disaccharide , stereochemistry , biochemistry , organic chemistry , optics , physics , in vitro , polymer , artificial intelligence , computer science
The lectin from Bowringia mildbraedii seeds crystallizes in the presence of the disaccharide Man(α1‐2)Man. The best crystals grow at 293 K within four weeks after a pre‐incubation at 277 K to induce nucleation. A complete data set was collected to a resolution of 1.90 Å using synchrotron radiation. The crystals belong to space group I 222, with unit‐cell parameters a = 66.06, b = 86.35, c = 91.76 Å, and contain one lectin monomer in the asymmetric unit.