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Two new functions have been recently developed based on the G-Rob systems. G-Rob system [1] was developed on protein crystallography beamline FIP-BM30A at the ESRF. G-Rob, A 6-axis robotic arm based system, is a fully integrated device for crystallography beamlines and laboratories. G-Rob is an “all in one” system. Thanks to its tool changer, it goes automatically from one application to another. A new tool for the robot (μ-Tweezer), a fully motorized inverted microscope (Visualization Bench) and an adapted environment has been developed to allow G-Rob automated in situ crystal screening and crystal harvesting. An image processing program has been developed which finds the well centre of the CrystalQuick X plates accurately. The centre of the well will be considered as the reference (0;0). A click on the centre of the crystal will save the coordinates referring to the centre (0;0) into a data base. This operation is done on the Visualization Bench independently from G-Rob and users can save as much as interesting crystals they have in their CrystalQuick X plate. By providing the plate to G-Rob, the same image processing program leads to an automated crystal centering and X-ray in situ screening [2] without any human intervention for a high-throughput diffraction analysis. The harvesting function allows grabbing crystals with the -Tweezer tool of the G-Rob system, which is a two-finger micro-gripping device. A semi-autonomous procedure has been developed in order to harvest a crystal from its crystallization drop by using a game pad. The robot while grabbing the crystal soaks it into an appropriate cryo-protectant solution before flash cooling the crystal in the 100K temperature nitrogen stream on the spindle position. Combining the goniometer function of the G-Rob system and the X-ray transparency of the ending elements of the μ-Tweezer tool, gives the ability to diffract crystals in the μ-Tweezer on 360° oscillation without dismounting or human manipulation of the crystal.

G-Rob with crystal-listing function for automatedin situdiffraction and with robotic crystal harvesting