Open AccessEffects of Light and External Solutes on the Catabolism of Nuclear-Encoded Stromal Proteins in Intact Chloroplasts Isolated from Pea Leaves
Author(s) -
Wataru Mitsuhashi,
Urs Feller
Publication year - 1992
Publication title -
plant physiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.554
H-Index - 312
eISSN - 1532-2548
pISSN - 0032-0889
DOI - 10.1104/pp.100.4.2100
Subject(s) - pisum , catabolism , glutamine synthetase , chloroplast , biochemistry , nitrite reductase , enzyme , sativum , biology , glutamine , amino acid , nitrate reductase , gene , botany
The catabolism of nuclear-encoded stromal proteins was investigated in intact chloroplasts isolated mechanically from pea (Pisum sativum) leaves. Glutamine synthetase, phosphoribulokinase, and nitrite reductase (quantified by immunoblotting) were more rapidly degraded in the light than in the dark. Furthermore, the degradation rates depended on exogenously supplied metabolites. For example, 2-oxoglutarate accelerated the catabolism of all three enzymes in chloroplasts incubated in the light, whereas oxaloacetate stabilized glutamine synthetase and at the same time destabilized the other two enzymes.