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Summary: The technique of autoradiography in the electron microscope has been used to determine the site of incorporation of tracer from tritium-labelled glucose, amino acids and bacteria by Entodinium caudatum and the ovine form of Epidinium ecaudatum caudatum. Both protozoa incorporated tracer from glucose principally into protozoal polysaccharide and intracellular bacteria. The results with the bacteria confirmed previous findings that Entodinium caudatum digested Bacillus megaterium rapidly in vesicles in the endoplasm, whereas Epidinium spp. lysed the bacteria in the medium before incorporation. These products were then taken up both by the protozoa and the bacteria attached to their surface. In the presence of Entodinium caudatum label from tritiated Escherichia coli was found principally in the endoplasm, whereas that from glycine was present in the ectoplasm also.

The Metabolism of Epidinium ecaudatum caudatum and Entodinium caudatum as Shown by Autoradiography in the Electron Microscope