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SUMMARY Successful infection of hop tissue culture with Pseudoperonospora humuli was achieved. Infection was obtained from hyphae emerging from surface- sterilized systemically infected stems and petioles. Externally, infection was characterized by sterile aerial hyphae and sporangiophores, and internally by intercellular mycelium and intracellular haustoria. Although a consider- able growth of mycelium into the agar medium was observed, it appeared to be dependent on the presence of host tissue. Attempts to establish axenic cultures of P. humuli were unsuccessful.

journal of general microbiology/journal of general microbiology

The Establishment of Hop Tissue Cultures and their Infection by Downy Mildew Pseudoperonospora humuli (Miy. &amp; Tak.) Wilson under Aseptic Conditions