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SUMMARY: Strains of Escherichia coliK12 carrying various R factors or the F factor were examined with the electron microscope. The F specific RNA phage MS 2 was added to differentiate the specific F type pili, which adsorbed the phage, from common pili, which did not. Even when phage was not added it was possible to identify F type pili because they were wider and generally longer than common pili. F type pili, but never common pili, often possessed terminal knobs whose presence on attached pili has not been previously described. The F type pili formed by E. coli carrying the different R factors or HfrH could not be morphologically distinguished from each other. Only one morphological type of common pilus was observed.

Morphological Features of the Pili Associated with Escherichia coli K12 Carrying R Factors or the F Factor