Open AccessA Dimeric Complex of Ruthenium: A New Inhibitor of Respiration-driven Calcium Transport in Escherichia coli K12
Author(s) -
Jane F. Gibson,
Robert K. Poole,
Martin N. Hughes,
J. E. Rees
Publication year - 1982
Publication title -
microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.019
H-Index - 179
eISSN - 1465-2080
pISSN - 1350-0872
DOI - 10.1099/00221287-128-9-2211
Subject(s) - ruthenium red , ruthenium , respiration , calcium , escherichia coli , chemistry , biophysics , cellular respiration , biochemistry , electron transport chain , biology , mitochondrion , catalysis , organic chemistry , botany , gene
Succinate-driven calcium uptake by everted membrane vesicles from Escherichia coli was sensitive to 2 mM-KCN and a dimeric, mixed valence complex of ruthenium, Ru2 (NH3)6Br5(H2O) (100 micro M), but relatively insensitive to 100 micro M-Ruthenium Red. The sensitivity of uptake to KCN and the slight inhibition caused by Ruthenium Red could be attributed to the different potencies of these compounds as respiratory inhibitors. Respiration was completely insensitive to the dimeric ruthenium complex, however, suggesting that this compound is an inhibitor of respiration-driven calcium transport.