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The nutritional requirements of 43 strains of Haemophilus influenzae isolated from clinical and normal flora sources were investigated. Two defined minimal media were developed by modifying the medium of Herriott et al. (1970):74% of the strains could grow on the minimal media and Herriott's medium; the remaining strains could not grow on any of these media.

Simplified Media for the Growth of Haemophilus influenzae from Clinical and Normal Flora Sources