Open AccessPhylogenetic Positions of Clostridium chauvoei and Clostridium septicum Based on 16S rRNA Gene Sequences
Author(s) -
Peter Kuhnert,
Selja E. Capaul,
Jacques Nicolet,
Joachim Frey
Publication year - 1996
Publication title -
international journal of systematic bacteriology
Language(s) - English
Resource type - Journals
eISSN - 1070-6259
pISSN - 0020-7713
DOI - 10.1099/00207713-46-4-1174
Subject(s) - clostridium septicum , biology , clostridium , 16s ribosomal rna , clostridiaceae , phylogenetic tree , gene , clostridiales , clostridium perfringens , genetics , clostridium difficile , microbiology and biotechnology , ribosomal rna , blackleg , bacteria , agronomy , brassica , antibiotics
The sequences of the 16S rRNA genes (rrs genes) of Clostridium chauvoei, the causative agent of blackleg in cattle, and the phenotypically related organism Clostridium septicum were determined. After amplification of 1,507-bp PCR fragments from the corresponding rrs genes, the sequences were determined in a single round of sequencing by using conserved region primers. A sequence similarity analysis of the sequences revealed the close phylogenetic relationship of C. chauvoei and C. septicum in Clostridium cluster I (M. D. Collins, P. A. Lawson, A. Willems, J. J. Cordoba, J. Fernandez-Garayzabal, P. Garcia, J. Cai, H. Hippe, and J. A. E. Farrow, Int. J. Syst. Bacteriol. 44:812-826, 1994), which includes Clostridium carnis, Clostridium perfringens, Clostridium botulinum, and Clostridium tetani. We found that 99.3% of the nucleotides in the genes of C. chauvoei and C. septicum are identical.