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Methods: The fecal microbiota of a subset of 17 patients who participated in the PUNCH CD study assessing RBX2660 (microbiota suspension) for recurrent CDI were characterized at 7 and 60 days post treatment using 16S rRNA gene sequencing in a post-hoc analysis. The patients (median 69.2 years; 65% female; 94% white) included 8 successes with 1 dose of RBX2660; 6 with 2 doses and 3 failures with 2 doses. All patients received a standardized 7-day course of vancomycin prior to dose 1 of RBX2660. A second dose was permitted within 10 days of recurrence; administered without antibiotic pretreatment. DNA sequencing was performed on an Illumina MiSeq platform. The 16S sequences were clustered into operational taxonomic units and used to determine within-sample diversity and taxonomic composition at each time point. Weighted Unifrac analysis was used to determine differences between sample diversity.

open forum infectious diseases

Increased Microbial Diversity Found in Successful Versus Unsuccessful Recipients of a Next-Generation FMT for Recurrent Clostridium difficile Infection