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This paper describes a simple, rapid, and validated reversed-phase high-performance liquid chromatographic method developed for the determination of 4 major bioactive constituents, namely, chlorogenic acid, ferulic acid, senkyunolide A, and Z-ligustilide in Rhizoma Cnidii extract. A Capcell Pak C18 chromatographic column (150 4.6 mm, 3 m) was used with mobile phases consisting of 0.1 formic acid, acetonitrile, and methanol at a flow rate of 0.8 mL/min and UV detection at 285 nm. Comprehensive validation of the method included evaluation of linearity, repeatability, recovery, and stability. Excellent linear behavior (r2 &amp;gt;0.99) was observed over the concentration range of 2100 g/mL for the compounds under investigation. Repeatability and accuracy were evaluated by intra- and interday assays; the relative standard deviation (RSD) values were 5.37 and accuracies ranged from 97.1 to 104.9. Recoveries of the compounds ranged from 94.2 to 104.2 with RSD values of 9.50. The developed method was successfully applied to the analysis of ethanolic extracts of Rhizoma Cnidii samples. As a result, the concentrations of chlorogenic acid, ferulic acid, Z-ligustilide, and senkyunolide A were determined to be 0.845.35, 0.451.65, 0.744.39, and 0.321.14 mg/g herb, respectively. Thus, the developed method was found to be accurate and reproducible and is considered suitable for the qualitative and quantitative analysis of Rhizoma Cnidii for bioactive compounds.

Simultaneous Determination of Phenolic Acids and Phthalide Compounds by Liquid Chromatography for Quality Assessment of Rhizoma Cnidii