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The Visual Immunoprecipitate (VIP®)for the Detection of E. coli O157:H7 in Foods, AOAC Official Method 996.09, has been modified to use a simplified plastic housing for the device. A methods comparison study was conducted to demonstrate the equivalence of this modification to the reference culture method. Three foods were analyzed. In total, valid results were obtained from 240 samples and controls. Results showed that the VIP for E. coli O157:H7 is equivalent to the reference culture methods for the detection of E. coli O157:H7.

journal of aoac international

Comparative Validation Study to Demonstrate the Equivalence of a Minor Modification to AOAC Method 996.09 [Visual Immunoprecipitate (VIP®) for E. coli O157:H7] to the Reference Culture Methods