English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Research has shown that traditional solvent extraction procedures used for the analysis of endogenous steroids often give inconsistent recoveries and results. However, a single-laboratory validation of a liquid chromatography/tandemmass specrometry method using 2 product ions per transition for progesterone, testosterone, and epi-testosterone in bovine liver and veal muscle showed accuracy and precision to within 23 at concentrations ranging from 0.5 to 2.0 g/kg. Homogenized samples were pretreated with methanol to denature endogenous enzymes. Following removal of methanol, samples were treated overnight with Helix pomatia -glucuronidase to deconjugate glucuronide conjugates. Alkali digestion of the samples in KOH solutions was done under shaking at 37C for 30 min. The digestate was extracted with methyl tert-butyl ether, and the extracts were cleaned by partitioning between acetonitrilehexane, followed by solid-phase extraction cleanup on silica cartridges. In bovine liver, average recoveries exceeded 54 for all analytes, and the within-run assay coefficients of variations were &amp;lt;6 and 13 for high (2.0 g/kg) and low (0.3 g/kg) analyte concentrations, respectively. In veal muscle, average recoveries exceeded 60, and the analysis of blind spikes gave accuracy estimates of over 85, with coefficients of variation (CVs) &amp;lt;15 for all analytes. The CVs for the multiple reaction monitoring ion ratios for all compounds were &amp;lt;22for all validation data. The method meets the requirements for confirmatory methods as outlined in 2002/657/EC. An analyst is capable of processing up to 20 samples within 5 days.

Alternative Methodology for the Analysis of Progesterone, Testosterone, and Epi-Testosterone in Bovine Liver and Veal Muscle