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A liquid chromatographic method is described for analysis of β-carotene in medical food. The nutrient is extracted from medical food without saponification by matrix solid-phase dispersion and quantitated by isocratic normal-phase chromatography with a Si 60 column and a mobile phase of hexane containing 0.125% (v/v) isopropyl alcohol. The limit of quantitation is 0.02 μg/mL at 436 nm. Standard response was linear over the concentration range of 0.02μ1.0 μg/ml(r2 = 0.99998). Recoveries were determined on a zero control reference material containing added β-carotene at various levels. Recoveries averaged 91.2% (n = 25) with coefficients of variation from 0.50 to 3.10%. The method provides a rapid, specific, sensitive, and easily controlled assay for analysis of β-carotene in fortified medical food. In addition, retinyl palmitate can be assayed simultaneously with an in-line fluorescence detector.

Analysis of β-Carotene in Medical Food by Liquid Chromatography with Matrix Solid-Phase Dispersion