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Direction derivatization in conjunction with liquid chromatography is described for assay of volatileacids induced by autoxidation of nonionic surfactants. The method is based on the reaction of acids with 2-nitrophenylhydrazine hydrochloride to produce nonvolatile hydrazine derivatives, which are separatedisocratically on a YMC-FA (C8) column within 10 min. In this study, formic acid was produced copiously in autoxidations, principally from C-C fission of hydroperoxides or free radicals. The method is superior with regard to stoichiometric measurement of acid formation, which is related to the extent of oxidation of nonionic surfactants.

Assay of Volatile Acids Induced by Autoxidation of Nonionic Surfactants by Liquid Chromatography with Direct Denvatization