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ampicillin, cefalothin, chloramphenicol, ciprofloxacin, erythro- mycin, kanamycin, rifampicin, tetracycline and trimethoprim. Susceptibility testing revealed no differences in the MIC of the tested antibiotics between C. jejuni NCTC 11168 cultured in standard media versus selective media. Comparison of the con- centrations of the antibiotics used in these media and their MICs for C. jejuni NCTC 11168 showed that the concentrations used in the media were substantially below the MIC values (Table 1). Gene expression analysis also revealed no differences in the expression of cmeABC or cmeDEF between C. jejuni NCTC 11168 cultured in MH media compared with any of the three selective media. Therefore, it is concluded that selective culture media used to isolate Campylobacter spp. inhibit competition by other bacteria and fungi, but do not induce overexpression of known efflux pump genes, for which some of these agents are substrates. Therefore, any antibiotic resistance observed in iso- lates is unlikely to be an artefact due to media composition as has been suggested previously.2

Antibiotics in media for isolation of Campylobacter spp. do not enhance resistance