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nue in the food industry are substantial. Globally the burden is even higher, and multinational outbreaks due to the global movement of contaminated foods are being described increasingly. The global food supply links nations and economies, emphasizing the need to view food safety with an integrated farm-to-fork lens. As predicted, advances in molecular techniques and information management have been transformative for food-borne disease investigation [2]. Almost 2 decades have passed since the Centers for Disease Control and Prevention (CDC) launched a national molecular subtyping system known as PulseNet, and since that time it has become an indispensable laboratory surveillance system for detection of multijurisdictional food-borne outbreaks. With PulseNet, public health authorities developed a unique system able to link bacterial isolates that could identify food-borne outbreaks dispersed far and wide geographically with only a few cases. Notable examples of PulseNet’s impact include elucidation of the association of Escherichia coli O157 and ground beef [3], as well as numerous produce-associated outbreaks [4,5].Not only food-borne disease investigations have been enhanced; investigations related to direct animal contact have improved as well [6, 7]. Indeed, PulseNet has facilitated more cluster evaluations, faster outbreak investigations, and timelier regulatory interventions and has undoubtedly prevented many illnesses

Whole-Genome Sequencing: Opportunities and Challenges for Public Health, Food-borne Outbreak Investigations, and the Global Food Supply