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Adult boll weevils (reared in the laboratory) were tested by dipping (4% or less in acetone solution) in the candidate chemical for 5 sec, air dried, and held at 30°C and 40 to 50% RH. Ten diet pellets were provided fresh daily for food and ovipositing. The candidate chemicals were also placed in the adult diet (0.5 or 0.25%) and fed to the newly eclosed weevils for 3 days. Eggs collected at 7 and 14 days posttreatment were divided and half held (2 weeks) for adult PI emergence. Three hundred and fourteen candidate chemicals were tested by feeding of which 13 were further tested by dipping.

Cotton, Laboratory Testing of Reproductive Inhibitors with The Boll Weevil 1981