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A laboratory study was conducted to determine if 2 strains of greenbugs differed in their susceptibility to 2 commonly used insecticides, parathion and chlorpyrifos. Greenhouse colonies were established from greenbugs collected from sorghum fields in Gove County and Haskell County, Kansas during the summer of 1989. The greenbugs in the Haskell County field had survived aerial applications of 0.75 lb (AI)/acre of parathion on 19 Aug and 0.5 lb (AI)/acre of parathion plus 0.25 lb (AI)/acre of Cygon on 24 Aug. The greenbugs in the Gove County field had not received any known insecticide treatment in 1989. Stock solutions containing 5 mg (AI)/ml of technical chlorpyrifos or parathion were prepared using histological grade acetone. Serial dilutions of each stock solution were made to obtain solutions with from 500 to 0.005 jig^Aiyml. The interiors of 1-dram glass vials were coated with 0.2 ml of each solution to form a series of vials with 0.001, 0.01, 0.1, 1, 10, or 100 u.g (AI)/vial. Control vials were treated with 0.2 ml of acetone. The trial was conducted on 9 Nov. Ten late instar greenbugs were placed in each vial and each concentration was replicated 5 times (50 greenbugs/concentration or 350 greenbugs from each colony for each chemical). Mortality was recorded after 7 h. Aphids incapable of coordinated movement were counted as dead.

Bioassay of Two Greenbug Strains with Parathion and Chlorpyrifos, 1989