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Two insecticidal tests were conducted in research greenhouses in Beltsville, MD. Tissue cultured plantlets were received from Apopka, FL and were grown in Promix BX for 3 mo in square plastic pots (10 × 10 cm at top). The first test was conducted in Jul ‘84. On Jul 20, 4 randomly chosen plants were each sprayed to the point of runoff with 25 ml of water. At the same time, another 4 plants were each sprayed with 25 ml of 0.4% neem seed extract. This concentration was prepared by diluting neem seed extract concentrate (sample A13-42845 (AN 4.57); Insect Chemical Ecology Laboratory, ARS, USDA, Beltsville, MD 20705) on a weight/volume basis with water. The concentrate contained 2300 ppm azadirachtin, an insecticidal constituent of neem seed extract. Because the neem seed extract contained ethanol, a third group of 4 plants was each sprayed with 25 ml 0.4% ethanol. Plants were allowed to dry for 24 h. They were then randomly placed in a cage under lights (16 h light: 8 h dark) with 50-75 adult leafminers for 24 h. Plants were then removed from the cage, leafminers were removed from the plants, and plants were returned to the greenhouse.

Control of Leafminer Using Neem (Azadiractha Indica a. Juss.) Seed Extract, 1984-85