Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes | Zendy

David H. Kwan | Zendy; Sabrina Ernst | Zendy; Miriam P. Kötzler | Zendy; Stephen G. Withers | Zendy

Open Access

Chemoenzymatic Synthesis of a Type 2 Blood Group A Tetrasaccharide and Development of High-throughput Assays Enables a Platform for Screening Blood Group Antigen-cleaving Enzymes

Author(s) -

David H. Kwan,

Sabrina Ernst,

Miriam P. Kötzler,

Stephen G. Withers

Publication year - 2015

Publication title -

glycobiology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.757

H-Index - 128

eISSN - 1460-2423

pISSN - 0959-6658

DOI - 10.1093/glycob/cwv031

Subject(s) - tetrasaccharide , enzyme , chemistry , biochemistry , oligosaccharide , antigen , glycoside , glycosylation , high throughput screening , glycosyltransferase , stereochemistry , biology , immunology , polysaccharide

A facile enzymatic synthesis of the methylumbelliferyl β-glycoside of the type 2 A blood group tetrasaccharide in good yields is reported. Using this compound, we developed highly sensitive fluorescence-based high-throughput assays for both endo-β-galactosidase and α-N-acetylgalactosaminidase activity specific for the oligosaccharide structure of the blood group A antigen. We further demonstrate the potential to use this assay to screen the expressed gene products of metagenomic libraries in the search for efficient blood group antigen-cleaving enzymes.

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