English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Tools annual

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Presents the access of premium content as premium feature

Premium Content

Global: Zendy Plus annual

Global: Zendy Free Plan

Since the initial studies of receptor-mediated endocytosis on LDL (1)(2) and asialoglycoprotein (ASGP) (3)(4), many internalized ligands and their receptors have been identified and characterized (5). It was soon recognized that these receptors could be exploited for pharmaceutical and medical imaging applications (6). In the case of the ASGP receptor (ASGP-R), delivery of diagnostic or therapeutic agents to hepatocytes was achieved by attachment of the agent to a variety of macromolecules possessing the terminal galactose signal necessary to bind the ASGP-R (6). These moieties include radioactive isotopes (7), drugs (8), and DNA (9).Considering the effort devoted to developing drug delivery systems utilizing the ASGP-R, surprisingly little effort has been devoted to developing simple, easy-to-use assays to measure this receptor activity in vivo. Much of the technology associated with receptor-based diagnostic agents has arisen in fields associated with magnetic resonance and single photon emission computed tomography imaging. One class of magnetic resonance compounds includes iron chelates, which appear to be absorbed by hepatocytes by an unknown biochemical mechanism (10). Saini et al. (11) and others have demonstrated the promise of ferrite particles as a magnetic resonance contrast agent for liver imaging. These commercially available micron-sized particles are taken up by the Kupffer cells of liver and do not measure ASGP-R activity.Sato et al. (12) reported on the ability of the ASGP-R to take up galactose-terminated, protein-coated ferrite particles in vivo. These particles are taken up preferentially by hepatocytes (70%) and largely avoid uptake by the reticular endothelial system. For a crystal-based material to bind the ASGP-R of hepatocytes, it must penetrate pores of the 100-nm fenestrae characteristic of endothelial cells that lie between the hepatocyte and the circulatory compartment (13). Particles smaller than 100 nm are needed to serve as hepatocyte-directed diagnostic agents.Josephson …

Hepatocyte Asialoglycoprotein Receptor Assay Using Stable Isotopes and Neutron Activation Analysis