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Urinary free cortisol is largely accepted as an accurate means of assessing daily cortisol secretion. A more precise definition of this marker should be “urinary free corticoids” (UFCs), because all competitive binding assays overestimate free cortisol in urine (1). Recently, it has been argued that urine volume may be a confounding factor because high fluid intake has been reported to significantly increase UFCs in healthy subjects (2), and an earlier study also stated that UFC concentrations are closely related to changes in urine volume in children (3). Conversely, a recent study published in Clinical Chemistry reported that UFCs are …

Urinary Free Cortisol Is Unrelated to Physiological Changes in Urine Volume in Healthy Women