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Inert thixotropic gel is commonly used in blood collection tubes for separation of serum or plasma from the cellular components of blood. Eichhorn et al. (1) in 1997 suggested that gel tubes (Sarstedt) used in their laboratory were generally reliable, but they found incomplete separation in 3.1% of tubes. Plain tubes are preferred for therapeutic drug monitoring because of the potential for binding of drugs to the gel (2). Smith (3) in 1985 and Hilborn and Krahn (4) in 1987 found that progesterone decreased with time as serum remained in contact with the separator gel. In contrast to these findings, we recently encountered an apparent increase in progesterone concentrations when blood was collected in glass serum separator tubes (SST® with Clot Activator; Becton Dickinson) and progesterone was measured by the Roche Diagnostics Elecsys® 2010.To further investigate these findings, we evaluated the effect of SST on several assays performed on the Elecsys 2010. All test samples (treated in compliance with the Human Investigation Committee) were assayed for progesterone, …

Effect of Serum Volume and Time of Exposure to Gel Barrier Tubes on Results for Progesterone by Roche Diagnostics Elecsys 2010