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We previously verified (1) the suitability of slide chemistry (2) with the Vitros 250 or 700 analyzers (formerly Ektachem 250 or 700, Johnson & Johnson) for analysis of urine samples. The sodium, potassium, urea, creatinine, phosphorus, and uric acid measurements, however, required the preparation of sample dilutions, which dramatically reduced analysis speed. The approach was further complicated by the fact that the diluting agent for sodium and potassium was the Urine Electrolyte Diluent (Johnson & Johnson), which could not be used for the other tests, which required dilutions with water.The present study was therefore carried out to attempt to standardize the dilutions with a single diluent and a single dilution. Because the diffusion of the sample in the spreading layer of the slides is important in slide technology, we tried an aqueous diluent selected with added surfactant. Preliminary studies (not shown) showed the possibility of ion determination with a Beckman diluent (wash solution, ref. 668601) without disturbances of the creatinine measurement. Therefore, to obtain the same surface tension with an aqueous solution of more defined composition, we tried a 0.15 g/L solution of Tween 20 (Merck, ref. 822184). This solution could be stored for 1 …

Single Dilution for Urine Assays on the Vitros 250 or 700 Analyzers