English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

PCR has the potential of extreme sensitivity, specificity, and diversity. As a consequence, transition of this procedure from research to routine application has been relatively rapid in recent years. This transition has, however, been hampered by the labor-intensive, high-skill requirements of existing protocols. Furthermore, as a number of quality-assessment exercises have demonstrated (1)(2)(3)(4)(5)(6)(7)(8), many of the existing “in-house” developed test procedures are of poor reproducibility in routine application. The introduction of a commercially produced test procedure has improved the reliability of testing, but the procedure has remained a relatively high-skill test method with only certain steps becoming semiautomated.The COBAS AMPLICORTM automated PCR system (Roche Molecular Systems) represents the first approach to full automation of the amplification and detection of nucleic acid targets (9). Evaluations of this instrument (10)(11) suggest that the sensitivity and specificity of the automated method are equivalent to those of manual methods, that intraassay sample carryover does not occur (9)(10), and that intra- and interassay variation using the automated system is low (9). However, there remains a need to evaluate the ability of the instrument, which is limited to 24 samples per thermal cycle amplification and 48 samples for the detection phase, to cope with both the work load and test diversity of a routine diagnostic laboratory. Moreover, the ability of the instrument to decrease the high labor and skill requirements of PCR and to decrease the overall cost of PCR needed to be further evaluated. We conducted a multicenter, international evaluation of the instrument from the standpoint of automation, work flow, and labor savings in laboratories differing in size, diversity of testing, and laboratory working practices.Five laboratories, chosen to represent a wide range of working …

Multicenter International Work Flow Study of an Automated Polymerase Chain Reaction Instrument