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Cryoglobulin is a thermoprotein precipitated at low temperatures in serum (1). It consists mainly of immunoglobulins, which form various complexes. They are classified into three types (I, II, and III) according to their composition (2). In recent years, there have been reports on the relationship between type II cryoglobulinemia and hepatitis C (2)(3)(4), which is being widely studied. The common method of detection of cryoglobulins is as follows. After blood is collected at 37 °C and serum separated, 5 mL of the serum is allowed to stand in a cryocrit tube at 4 °C for 2–7 days, and formation of the precipitate is confirmed visually. When a precipitate is found, the sample is centrifuged at 4 °C; the supernatant is removed; cold physiological saline or phosphate buffer (pH 5.5–7.0) is added; and the mixture is heated at 37 °C. If the precipitate redissolves after heating, …

Gel Diffusion Procedure for the Detection of Cryoglobulins in Serum