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The efficacy of Beauveria bassiana spore applications for control of mole cricket nymphs in turf was investigated in a field trial. The objective of this test was to compare the efficacies of one high rate application to multiple low rate applications of B. bassiana. The checks included Talstar Flowable and an untreated check. Plots, 20 × 20 ft, were established on old fairway at Sea Trails Plantation in Brunswick Co., NC, an area with a history of mole cricket infestations and signs of damage present at the time of application. The test was set up as a RCB with four replicates of each treatment. The initial treatment applications were made on 6 Jul using a CO2-powered backpack sprayer calibrated to deliver 30 gpa. On this date, the high rate of B. bassiana, Talstar, and the first application of the repeated low rates of B. bassiana were made. The high rate of B. bassiana delivered 2 × 10 spores/acre, while each of the four applications at the low rate delivered 5 × 10 spores/acre, for a cumulative number of spores/acre equal to the high rate. The subsequent applications of the low rate of B. bassiana were made on 21 Jul, and 1 and 17 Aug. Talstar was applied at the label rate of 10 oz/1000 ft. The golf course irrigation system provided approximately 0.5 inch of water to the test site immediately following all treatment applications. The air temperatures were 86oF on 6 Jul, 82oF on 21 Jul, 76oF on 1 Aug, and 85oF on 17 Aug. Soil at the test site was classified as Foreston fine sand with a pH of 3.9 and 0.86% humic matter. Soapy water flush sampling at the test site indicates that the fairway population was 93% tawny mole crickets and 7% southern mole crickets. Plots were evaluated for fresh mole cricket surface damage on 11, 17, 23, 31 Aug, and 7 Sep using the damage grid evaluation method of Cobb and Mack (1989). A one square meter grid divided into nine subsections was randomly placed in each plot and a damage rating was assigned based on the occurrence of surface damage in each of the nine subsections (with 0 = no damage and 9 = severe damage). Five ratings were made for each plot on each of the evaluation dates. The actual damage means for the five ratings are reported in the Table. All data were transformed √(x + 0.5) prior to ANOVA and means separation by LSD t-test (P ≤ 0.05).

EFFICACY OF SINGLE AND MULTIPLE APPLICATIONS OF BEAUVERIA BASSIANA FOR CONTROL OF MOLE CRICKET NYMPHS IN TURF, 2005