English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Plus monthly

Global: Zendy Tools annual

Global: Zendy Tools monthly

Global: Zendy Free Plan

Merozoite surface protein-1 (MSP-1) is one of the several proteins on the surface of the asexual merozoite of malaria parasites. It undergoes a two stage proteolytic cleavage to form a C-terminal 19kDa fragment or PfMSP119 that is used for red blood cell invasion. A homologue of PfMSP-119 of Plasmodium falciparum called PvMSP-119 has been identified in Plasmodium vivax. Recently, attention has been focused on this C-terminal cysteine-rich region as an important determinant of haplotypes that characterize the malaria parasite in endemic areas. In this study, sequence specific primers were used to determine the PfMSP-119 haplotypes from field samples and PvMSP-119 haplotypes from both field and Aotus adapted parasites. 163 PfMSP-119 samples from Kisumu-Kenya were polymerase chain reaction (PCR) typed at position 1644 in the first epidermal growth factor (EGF)-like domain followed by sequencing of a random selection of positive samples so as to evaluate molecular changes at positions 1691, 1700 and 1701 in the second EGF-like domain. 121 selected samples gave bands, with 80% typing as GAA (E) and 20% as CAA (Q) at position 1644. From computer-alignment sequences, samples 96B216 confirmed as the Uganda-PA haplotype (E-KNG), 96B209 as the PfMAD20 haplotype (E-TSR), 96B208 as the PfK1/Wellcome haplotype (Q-KNG), 96B183 as the Uganda-PA haplotype (E-KNG) and 96B017 as the E-TSG haplotype. Next, genotyping and sequence analyses of the PvMSP-119 fragment against reference sequences from GenBank demonstrated isolates as belonging to either one or the other of two parental haplotypes identified as Belem and Sal-1 strains. Comparison of PfMSP-119 and PvMSP-119 fragments demonstrated highly conserved cysteine amino acids in the two EGF-like domains.

Characterization of the MSP-1 Protein in Field Samples of Plasmodium falciparum and its Homology to the Plasmodium vivax MSP-1 Protein