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Direct immunofluorescent staining of centrifuged viral transport medium (CVTM) was compared with conventional cell culture for the detection of varicella-zoster virus (VZV) in 87 dermal lesions from 84 patients. A total of 21 (24%) were positive for VZV; 8 (38%) of these were positive by culture and CVTM, 13 (62%) by CVTM alone, and none by culture only. Virus cultures were positive for VZV in an average of 9.1 days (range, 4-20 days). CVTM, using cytocentrifugation, is more sensitive and rapid than conventional cell culture for the detection of VZV in cutaneous specimens.

american journal of clinical pathology

Comparison of Virus Culture and Direct Immunofluorescent Staining of Cytocentrifuged Virus Transport Medium for Detection of Varicella-Zoster Virus in Skin Lesions