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Since the investigation of semi- or non-conductive fibers in a scanning electron microscope (SEM) is often a critical problem because of charging issues, this study attempted to find out conditions that may offer high-resolution images. Four factors: beam current, acceleration voltage, vacuum, and sample preparation, were varied to investigate carbon and natural bamboo fibers by minimizing the charging problem. Results indicated that, unlike carbon, natural bamboo fiber was poor in SEM investigation without coating either in environmental, low or high vacuum due to charge formation. However, investigation in a low vacuum below 5 kV was proven to be very effective of uncoated bamboo fiber.

An Investigation of Charging Effects by Different Factors on Carbon and Natural Bamboo Fibers in High-Resolution Scanning Electron Microscopic Imaging