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In this study, the essential oil of leaves  Eucalyptus globulus  (Myrtales: Myrtaceae) was analyzed by gas chromatography/mass spectrometry (GC/MS) and their potential insecticidal against the olive fruit fly  Bactrocera oleae  (Diptera: Thephritidae) was investigated. The essential oil yield was 0.36 ± 0.03% (w/w). A total of 41 compounds (99.99%) were identified. (-)-spathulenol (32.66%), o-cymene (28.30%) and (+-)-cryptone (12.13%) were major constituents. The toxic impact of essential oil of  E. globulus  was assessed against  B. oleae  adults through two toxicity tests (contact and fumigation).  B. oleae  was more susceptible to  E. globulus  and adult mortality increased according to concentration and exposure time. In the contact toxicity, the lethal concentration LC 50  values were 0.24 µL/cm 2  and 0.15 µL/cm 2  in 24 h and 48 h, respectively and LC 90  values of 0.64 µl/cm 2  and 0.33 µL/cm 2  were obtained in 24 h and 48 h, respectively. In fumigation assay on olive fruit fly adults, LC 50  values were 14.05 μL/L air and 5.71 μL/L air at 24 h and 48 h, respectively and LC 90  values of 51.53 μL/L air and 21.64 μL/L air at 24 h and 48 h, respectively. The findings of current study show that use of essential oil of leaves of  E. globulus  against  B. oleae  is considered as an eco-friendly alternative natural insecticide.

journal of essential oil bearing plants

GC/MS Analysis of  Eucalyptus globulus  L. (Myrtaceae) Leaves Essential Oil from Algeria and their Insecticidal Activity Against Adults of  Bactrocera oleae  (Rossi) (Diptera; Tephritidae)