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Vitamin D intakes are concerningly low. Food-based strategies are urgently warranted to increase vitamin D intakes and subsequently improve 25-hydroxyvitamin D (25(OH)D) concentrations. This acute randomised three-way crossover study investigated the efficacy of vitamin D biofortified pork derived from pigs exposed to UVB light to increase serum 25(OH)D 3  concentrations, compared to a dose-matched vitamin D 3  supplement and control pork in adults ( n  = 14). Blood samples were obtained at baseline and then 1.5, 3, 6, 9 and 24 h postprandially. There was a significant effect of time ( p  &lt; 0.01) and a significant treatment*time interaction ( p  &lt; 0.05). UV pork and supplement significantly increased within-group serum 25(OH)D 3  concentrations over timepoints ( p  &lt; 0.05) (max. change 0.9 nmol/L (2.2%) UV pork, 1.5 nmol/L (3.5%) supplement, 0.7 nmol/L (1.9%) control). Vitamin D biofortified pork modestly increased 25(OH)D 3  concentrations and produced a similar response pattern as a dose-matched vitamin D supplement, but biofortification protocols should be further optimised to ensure differentiation from standard pork.

Bioavailability of vitamin D biofortified pork meat: results of an acute human crossover study in healthy adults