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The antibiotic amoxicillin (AMX) is a semisynthetic aminopenicillin, classified as an β-lactam antibiotic. This work aims to evaluate the AMX degradation (190 mg L –1 ), in aqueous medium, applying photo-Fenton ([TOC] 0  = 100 mgC L −1 ; F H2O2  = 3.27 mmol min −1 ; [Fe 2+ ] = 0.27 mmol L −1 ; pH = 3.0; T = 40°C) and acid hydrolysis processes. Along the experiments, samples were withdrawn and analyzed by a total organic carbon (TOC) analyzer and a liquid chromatography system coupled to diode array (HPLC-DAD) and mass spectrometry (HPLC-MS) detectors. The hydrolysis process proved to be less efficient, because AMX removals greater than 80% were observed only after 24 hours of reaction (pH 2). Conversely, the photo-Fenton process removed completely AMX in just 20 minutes, reaching 85% of TOC removal in 2 hours. Finally, the AMX aqueous solutions treated by the studied processes was also evaluated in respect of its toxicity to some microorganisms, applying two antimicrobial susceptibility tests: disk-diffusion and broth microdilution methods. It was observed that the AMX aqueous solutions, pretreated by the photo-Fenton process, for just 7.5 min of reaction time, did not inhibit the microorganisms growth. The obtained results show that the photo-Fenton process was able to degrade AMX, in a relatively short time, and that the generated degradation products did not inhibit the microorganisms growth, when compared to acid hydrolysis process. Thus, it was verified the potential application of the photo-Fenton system as a pretreatment step to conventional biological oxidation processes for the treatment of industrial wastewaters.

Degradation of amoxicillin applying photo-Fenton and acid hydrolysis processes with toxicity evaluation via antimicrobial susceptibility tests