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The upflow anaerobic hybrid (UAHB) reactor combines the advantages of a upflow anaerobic sludge blanket (UASB-type) reactor and an anaerobic filter in a single compartment. A novel configuration of the UAHB reactor, composed of two three-phase separators (3PHS), was proposed to evaluate the biogas production in the moving and fixed bed in the treatment of synthetic sewage at a temperature range of 14–21 °C and hydraulic retention time (HRT) of 12, 10 and 8 h. The bench-scale reactor was operated in three different phases with organic loading rate (VOLR) of 0.6 (0.3–0.7), 0.7 ± 0.2, and 1.1 ± 0.1 kg COD m −3  d −1 , respectively, for 225 days. The average removal efficiency of chemical oxygen demand (COD t ) was 78 (42–89)%, and the total biogas yield was 3090 (1704–4782) mL d −1 , with 66% of the lower 3PHS (moving bed) and 34% of the upper 3PHS (fixed bed). However, no significant difference was observed between the biogas yield on the 3PHS ( p -value = 0.5048), thus confirming the influence of temperature in the biogas production. The average percentage of methane was 76 (60–82)% for both beds, and the filter media increased the production by 21%. Thus, it can be concluded that the fixed bed suppressed the instability of the moving bed regarding the biogas production and contributed to the final quality of the effluent.

Effects of temperature and HRT on biogas production in moving and fixed bed of a novel upflow anaerobic hybrid (UAHB) reactor