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Sugarcane is the most traded crop in the world, with Brazil being the world’s largest producer. Sugarcane processing generates up to 28% of sugarcane bagasse (SB) from the entire plant, with only 50% of it used for energy generation. SB is a lignocellulosic biomass that can be converted into biogas. However, the optimization of pretreatment process parameters is essential for its successful scaling up. This study evaluated the effect of mild alkaline pretreatment of SB using NaOH and KOH at concentrations of 1–10% and exposure time of 1–12 hours) on the biochemical methane potential (BMP) under mesophilic temperature. The central composite rotatable design (CCRD) was applied as statistical tool to generate optimal operating pretreatment conditions. The tests were performed in triplicates totalizing 84 batch bottles. The BMP of the untreated SB varied between 297–306 L N  CH 4  kg VS −1  while the BMP of the pretreated samples with NaOH and KOH were 19% and 20% higher. The optimized conditions were NaOH at 7.7% and KOH at 8.3% KOH for 12 hours. However, the range indicated by the statistical design with CCRD revealed that there was no statistical difference in terms of methane yield when concentrations between 4–10% NaOH and 6–10% KOH during 12 hours were applied, when compared to the specific optimized points. The optimization of the pretreatment parameters demonstrated to be a key-factor to improve the anaerobic digestion of lignocellulosic substrates, leading to a less chemically dependent and more sustainable approach, while allowing a more profitable process.

Optimization of chemical solution concentration and exposure time in the alkaline pretreatment applied to sugarcane bagasse for methane production