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A bacterial strain ASLT-13 was successfully isolated from activated sludge and identified as  Pseudomonas amygdali . Gas chromatograph-mass spectrometer (GC-MS) analysis indicated that strain ASLT-13 could completely mineralize di 2-ethyl hexyl phthalate (DEHP). DEHP was first metabolized from the longer side chain of the benzene ring into shorter branches (Phatlalic mono-esters) like Dibutyl phthalate (DBP) under the action of degrading genes. DBP was then converted into di-methyl phthalate (DMP), and then hydrolysed to phthalic acid (PA). PA was eventually converted to CO 2  and H 2 O through the TCA cycle. The optimal conditions for immobilization were the sodium alginate (SA) concentration of 6%, CaCl 2  concentration of 5%, ratio of bacteria and SA of 1:1, crosslinking time of 6 h. Bacterial quantity and community structure in sequencing batch reactors (SBRs) was investigated by q-PCR and high-throughput sequencing. The results indicated that DEHP removal efficiency was significantly enhanced by immobilization.  Arthrobacter, Acinetobacter ,  Bacillus  and  Rhodococcus  were the predominant genera for DEHP degradation. This study suggested that the cell immobilization technology had a potential application in DEHP wastewater treatment.

environmental technology

Biochemical pathways and associated microbial process of di-2-ethyl hexyl phthalate (DEHP) enhanced degradation by the immobilization technique in sequencing batch reactor